Gfox cells are CrFK cells that have been engineered to over-express CD134 [6].
The effect of DSS1 over-expression on cell proliferation and cell cycle progression was examined in breast cancer cells that had been engineered to over-express DSS1 (MCF7DSS1 or MDA-MB-231DSS1 MDA-MB-231DSS1 MDA-MB-231DSS1Additional file 2: Figure S2).
In order to investigate these questions, A549 cells were engineered to over-express the type 2 cannabinoid receptor (CB2R) using a self-inactivating lentiviral vector.
These transgenic mice were engineered to over-express in B lymphocytes a TRAF2 mutant that mimics TRAF1 and the anti-apoptotic protein Bcl-2.
For preparation of rBCGacr, a Mycobacteria - Escherichia coli shuttle plasmid pSD5.pro [33], [33] was engineered to over-express α-crystallin gene (Rv2031c) under the transcriptional control of the promoter of hsp65 gene of M. leprae.
Briefly, the plasmid (pSD5.pro) was engineered to over-express Ag85C along with its native signal sequence under transcriptional control of the promoter of M. leprae gene encoding heat shock protein 65 (hsp65).
To functionally link hTRM9L expression to tumourigenicity, we used a gain of function strategy where SW620 and HCT116 cells were engineered to over-express hTRM9L.
Almost all mouse models of AD are engineered to over-express human APP to such an extreme extent that animals show pathology within months of birth.
MCF-7 cells were engineered to over-express the glycosylated clusterin isoform using Gateway Technology Invitrogen Corporationn, Carlsbad, California) according to the manufacturer's protocol.
Briefly, LNCaP cells were engineered to over-express wild type human AR and to express an ARE2-PB-Luc reporter (LNAR cells).
Biological production of 3-HP from glycerol has been successfully demonstrated in several bacteria such as Escherichia coli, Klebsiella pneumoniae and Pseudomonas denitrificans, which are engineered to (over)express glycerol dehydratase (GDHt, coenzyme B12 -dependent), glycerol dehydratase reactivation factors (GdrAB), and/or aldehyde dehydrogenase (ALDH, NAD+-dependent) [ 4– 8].
