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The plasmids have been engineered to contain each of the appropriate globin genes, linked in tandem to artificial galactose promoters.
Existing methods of circRNA expression thus typically involve genes that have been engineered to contain sequence elements that promote back-splicing.
This surrogate RNA has been engineered to contain sequences from the 5′UTR and 3D regions of FMDV targeted by many molecular assays (conventional RT-PCR, real-time RT-PCR and RT-LAMP).
For example, corn has been engineered to contain the insecticidal protein, Bt, a protein that protects plants by killing insects that ingest it.
Several proteins have been engineered to contain a Furin consensus motif in their reactive site loops to thereby inhibit Furin.
All HCF-1 mutants have been engineered to contain a FLAG tag and an HA tag at 5' of the transcription start site.
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However, bacterially derived non-replicating minicircle DNA, which is created by removal of plasmid backbone DNA through intramolecular site-specific recombination within a minicircle producer plasmid, can be engineered to contain predominantly human sequences with a low incidence of immunostimulatory CpG-containing motifs [ 1].
The corn is engineered to contain a gene that is lethal to a common pest.
Golden rice is engineered to contain three new genes that together cause rice to produce beta carotene.
The attraction of algae would seem to lie in the possibility that, since they photosynthesise, they could be engineered to contain the whole sunlight-to-fuel process in one genetically engineered package.
The repair genes were engineered to contain an HA epitope tag at their C-termini.
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