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Surfaces have been engineered at the molecular level in terms of the presence of specific biological factors, their arrangement on a surface, and their diffusivity to elicit specific lymphocyte fates.
In this mutant, the cysteine has been engineered at the first arginine of S4, producing a slowly activating K+ current when compared to WT Kv7.1, which is reminiscent of the slow IKS activation kinetics (at +40 mV, τact = 1,509±60 ms for R228C, compared to τact fast = 80±3 ms and τact slow = 736±44 ms for WT Kv7.1, n = 8; p<0.01) (Figure 6).
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Hotspots were engineered at the junctions as indicated by the finite difference time domain calculation.
For proper export of jerdostatin, a secretion leader sequence was engineered at the protein's N-terminus.
Wetting studies shows that surfaces of these SAMs are stable for months, thus providing stable model surfaces that can be engineered at the molecular level.
Gradients can thereby be engineered at the cellular scale and this approach has allowed to disentangle roles of the nucleus and protrusion activity in setting cell directions.
Charge- or exchange-bias-mediated magnetoelectric coupling that relies on uncompensated interfacial charges or spins can also be engineered at the nanoscale.
As part of a general study into the impact of quaternary structure on enzyme function, a library of 31 point mutations were engineered at the dimer dimer interface of the homotetrameric 8-barrel protein, N-acetylneuraminate lyase (NAL, EC 4.1.3.3).
The human IgG signal peptide and a Kozak sequence were engineered at the 5'ends of the VK and VH sequences.
Epitope tags (3X Myc or HA) were engineered at the amino terminus of wild type and P58S VAP using PCR.
Additionally, a covalent interchain bond was engineered at the C-termini of the GR1 and GR2 domains to further stabilize the ccFv construct.
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