A loop design was employed for microarray analysis [42], with each CNS region compared to another region belonging to the same species, on multiple arrays per species.
While some variability was expected, given that separate dissections were employed for microarray analysis and QPCR experiments, the general pattern and degree of difference detected by the two techniques were very similar, and results agreed in 32/38 cases (84%).
All unique annotated transcripts (15,385; see Results), excluding those annotated only with Unigene (2,549), were employed for microarray design.
After further clustering by proteome mapping, a total of 1,346 Isotigs (1,196 showing the same annotation with all 5 fish species) were filtered out, yielding a total of 15,385 unique annotated transcripts, which were employed for microarray design.
Unfortunately, while this type of system has been employed for gene microarray analysis, it is unclear whether it will be effective in the analysis of mass spectral data, which unlike microarray data, is comprised of continuous measurement operations.
In the present study, oligonucleotide microarray has been employed for a better understanding of gene expression signatures.
In a few cases, microarray technology has been employed for sequence-driven screening of metagenomic DNA and libraries.
At present, several experimental techniques have been employed for identifying DNA-binding proteins, such as filter binding assays, genetic analysis, chromatin immunoprecipitation on microarrays, and X-ray crystallography.
ZnO films annealed at T ⩽ 300 °C show the most promising surface features to be employed for DNA microarray preparation, i.e. high density of binding sites (hydroxyl groups), smooth and homogeneous surfaces, high optical transmittance in the visible spectral range suitable for detection using fluorescence, and easy handling during preparation procedures.
The fluorescent dye-labelled cDNA and hybridization strategy was employed for the microarray assay.
