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TALEs have also been efficiently fused to the TET family of active DNA demethylases and drive gene expression in targeted sequences by actively removing DNA methylation [ 77, 78].
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Furthermore, metallothionein has been efficiently expressed without being fused to a carrier protein, achieving high mRNA levels, mercury resistance and accumulation.
Here we show for the first time that metallothionein can be efficiently expressed in bacteria without being fused to a carrier protein to enhance mercury bioremediation.
When tags (receptor-binding proteins or cell-penetrating peptides) are fused to PDs, they efficiently cross the intestinal epithelium and are delivered to the circulatory or immune system.
Protein stability can be efficiently controlled by conditional degrons, which induce target protein degradation at restrictive conditions.We used the yeast Saccharomyces cerevisiae for development of a conditional, bidirectional degron to control protein stability, which can be fused to the target protein N-terminally, C-terminally or placed internally.
The fact that LC3 II and p62 levels did not decrease over time suggests that vacuoles were not efficiently fused with the lysosomes.
G9a was fused to mVenus at the C-terminus.
Here, ATPS is fused to APR.
Rice NH proteins are fused to LexA and TGA proteins fused to B42AD.
Rice NH proteins are fused to YFPN and TGA proteins fused to YFPC.
Rice RH proteins are fused to YFPN and NH proteins fused to YFPC.
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