Its strength is that it is done in larval zebrafish where Purkinje neuron activity can be correlated with motor behavior.
A similar search was done in mouse, rat, zebrafish and chicken genomes.
This was done in embryos, in early larvae and in organs of adult zebrafish.
Embryos and early larvae were the prevalent zebrafish life stages in these studies, with exposure being done in multiwell-plates, often with several individuals in the same well.
Further studies are required to quantify the respective role of Ca2+ from the extracellular fluid and the SR to the Ca2+ transient responsible for contraction in trout cardiomyocytes, as it has been recently done in zebrafish (11).
Around that time, Mike met Len Zon, who was doing screens in zebrafish to identify genetic mutants that affected the cell cycle.
In this case, the characterization of effects was done by extraction of zebrafish lipids followed by thin-layer chromatography.
Identification of RNase T2 genes was done by BLAST searches in the zebrafish (Danio rerio) genome (version Zv7, available through the National Center for Biotechnology Information (NCBI),.
The randomization was done using all the human homolog of zebrafish (8056 human UniGene identifiers) generated by mapping all the zebrafish genes in the microarray to HomoloGene database as described earlier.
The point of eye rotation, which has been described in zebrafish does not pose a problem for the relative annotation of expression patterns during eye cup formation.
