Since samples at different gestational ages were dissected from different individuals, gene expression associated with this variable also corresponds to inter-individual variability and must be interpreted accordingly.
For each biological replicate more than 20 embryos were dissected from different F1 females.
6, 24, or 48 hours after injection (n = 3) the animals were euthanized and tissues were dissected from twelve different organs: kidney, spleen, liver, colon, brain, heart, lung, pancreas, muscle, small intestine, gonads, and bone marrow.
Forty-eight hours after injection (n = 3), the animals were euthanized and tissues were dissected from 6 different organs: liver, spleen, kidney, lung, heart, and brain.
Salivary gland tissue was dissected from ~400 aphids of different life stages (predominantly fourth instar alates and alate adults) reared on cabbage (B. oleraceae).
For analysis of the most stable genes during male and female gametophyte development, ovaries and anthers of both accessions were dissected from flowers at four different stages of development before anthesis, as previously described by Rodrigues et al. (2003).
Small pieces of spleen or lymph node, approximately 1 mm3 in size, should be dissected from two or three different areas and ground lightly in one or two drops of water on a glass slide using the end of a glass rod. 2. Using a fine-bore pipette or loop, transfer a drop of suspension to a Formvar-coated copper grid and allow to adsorb for ten seconds to several minutes.
Mice cortex and hippocampus were dissected from C57BL/6 J mouse at different stages indicated.
The different tissues were dissected from mature dry beans.
Gill tissues from 6 different animals were dissected from -80°C preserved animals and processed for total RNA extraction using the RiboPure tm) kit (Ambion(r), Austin, TX).
