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Historically legumes have been difficult to transform and regenerate.
Recombineering was recently adapted for Drosophila, through the use of the P[acman] vector and ϕC31-mediated transgenesis., Historically it has been difficult to transform Drosophila with large pieces of DNA (> 30 kb).
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And if history is a guide, it is difficult to transform excitement over a concert into a coherent social movement.
They can hardly be used without adjoint code and they are difficult to transform from one simulator to another.
The computational efficiency of gradient-based algorithms is the highest but they are difficult to transform from one simulator to another.
However our system differs in several respects from the MMEC model: (i) We employed human epithelial cells, which are difficult to transform [32], [33].
However, An. gambiae is known to be difficult to transform, highlighted by the scarcity of publications [20] [24], and efficiencies are unlikely to reach the levels reported for D. melanogaster or Ae. aegypti.
For the many different types of microarray platforms, however, it is difficult to transform raw data so as to accord with any one of the existing frameworks, precluding reliable comparisons among dissimilar data sets.
Firstly, some thermophilic bacteria are difficult to transform due to the unique features of their cell envelope, the formation of endospore and the low permeability of plasma membrane [4].
Such results are difficult to transform into straightforward treatment recommendations or patient information.
For this purpose, we need to know which cellulase genes to transform into the host genome because it is difficult to transform many genes into a genome.
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