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Also, how these two dimensions of inclusion and closure play out has been differentiated for modes of governance.
To examine the functional properties of dopaminergic neurons derived from ESCs/NSCs cultured in defined medium, we transplanted cells that had been differentiated for 20 days (after the NSC stage) into the striatum of rats.
In striking contrast to the mC2C12 cells, C2C12 cells that had been differentiated for nine days (dC2C12) did not undergo death when injected with bovine or yeast cytochrome c (Fig. 1a, b).
We performed an unbiased FACS- and image-based screen using a collection of 190 characterized monoclonal antibodies to cell surface markers on cell cultures at distinct phases of neural induction by SFEB: naïve hESC, EB-rosette, manually picked and expanded NSC, and NSC that had been differentiated for 3 weeks to mixed populations of neurons and glia (Fig. 1, Fig. S2).
When the cells had been differentiated for 20 to 55 days, they were dissociated into single cells.
We first performed immunocytochemistry to determine the levels of hCx43 and β-catenin proteins in hNPCs derived neurospheres, which had been differentiated for 2 h.
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Nsphs were differentiated for five days in differentiation medium [DMEM/F-12, N2, 1% penicillin/streptomycin and 0.5% FBS (Invitrogen)].
To investigate the effect of glutamate on oligodendrocyte differentiation, pre-committed neurospheres were differentiated for 3 days in the presence of glutamate receptor agonists.
Cells were differentiated for 4 days, with fresh NPC differentiation media added at day 2. Live cell imaging of NPCs and NPC-derived neural progeny in culture was performed on an Olympus (Center Valley, PA, USA) IX70 inverted microscope.
Bone marrow cultures were differentiated for 1 week with additions of fresh differentiation medium at days 3 and 6.
In an initial experiment, H1 HESCs were differentiated for 30 days with RNA samples taken before and after differentiation.
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