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A recent scientific paper showed that all 150 drugs tested at the cost of billions of dollars in human trials of sepsis failed because the drugs had been developed using mice.
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Separate B6.CAST control (B6C) and HG.CAST control (HGC) strains were developed using mice from the MMU2 experiment.
The blot was developed using mouse anti-human transferrin Mab (Abcam).
Over the last decade, whole-genome approaches for the discovery of gene function and new models of human disease have been developed using the mouse.
Transgenic mouse models that generate spontaneous mammary tumors have been developed using promoters such as the mouse mammary tumor virus (MMTV) promoter to drive oncogenes, including polyoma middle T antigen (MMTV-PyMT) and ErbB2/Neu (MMTV-Neu) (for review see [ 5]).
Several transgenic mice have been developed using downstream transcriptional factors Gli1, Gli2, and Shh [155]– [157] The inducible expression of Gli2 in the skin results in BCCs after a few weeks.
Transgenic mice have also been developed using a yeast artificial chromosome from this region (YAC 152F7).
The primary mouse antibody (αSMA) was developed using a mouse-on-mouse detection kit.
This model was developed using 129/Sv mouse gene sequence cloned into BALB/cByJ blastocysts, and are maintained on a mixed background (129 BALB) [42].
The reaction was developed using anti-mouse poly-alkaline phosphatase (AP) and anti-rabbit poly-horseradish peroxidase (HRP) polymerization technology.
Xenografts were developed using RAG1-/ mice by injection with WM239 or SW480 cells and mice were treated with 20 mg/kg/day of cyclophosphamide in their drinking water for up to 18 days.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com