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Recently, given the success of exome sequencing in identifying human disease-causing variants, hybridization-based exome capture systems have been developed for mouse and are now commercially available [ 12, 13].
Data integration and interpretation with a cross-species transcriptomic analysis brings its own challenges and although complete physical maps have been developed for mouse, macaque and swine genomes to support genome sequencing and comparative genomics, functional annotation to date is mostly based on human, mouse, and rat literature.
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In recent years, touchscreen-based cognitive tasks have been developed for mice and rats to provide a better translational approach across species for further understanding the cognitive impairments observed in various neuropsychiatric disorders and for testing potential pharmacological interventions.
Soluble RAGE made from the extracellular two thirds of the receptor was developed for mouse studies, but limited quantities are currently available.
Our model was developed for mouse and human using species-specific parameters.
A secreted protein database was developed for human, rat and mouse, but unfortunately this database has not been updated since 2006 (http://spd.cbi.pku.edu.cn/) (16), and another database, LOCATE, describing the membrane organization and subcellular location including secreted proteins was developed for mouse and human only (http://locate.imb.uq.edu.au/) (17).
Although the current model is developed for mice, it can easily be applied to virtually any animal model and for humans, since most mammals follow the canonical PHD2-HIF1α based pathway for erythropoietic stimulation.
A soft agar colony assay has been developed for the B16 mouse melanoma and the Lewis lung tumour.
Recently, a multiplexed activation of endogenous Oct4, Sox2 and Ilirn genes by an inducible Tet-on CRISPR/dCas9 system has been developed for human and mouse cells.
ER activity assays have been developed for fish and mice; however, most reporter constructs are designed to act in certain tissues exclusively (such as liver or brain) (Brion et al. 2012; Kurauchi et al. 2005) or have used a bioluminescent reporter (such as luciferase) that has limited spatial resolution (Ciana et al. 2003; Legler et al. 2000).
A new range of nonhuman fluorescence in situ hybridization (FISH) probes has been developed for visualizing pig, chicken, and mouse chromosomes.
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