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Phonation threshold pressure values reported in the literature have largely been determined with use of one of three consonant-vowel sequences, /pi/, /pae/, and /pa/; however, it is not currently known if vowel choice influences phonation threshold pressure values.

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The linear rate of healing was determined with use of computerized planimetry.

The total phenolic contents were determined with use of Folin Ciocalteu method.

The reducing ability of extract was determined with use of FRAP analysis, and it was determined by the standard method.

In this study, the position of the mandibular foramen was determined with use of CBCT and compared in patients with normal occlusion, skeletal class II and III malocclusion.

Union rates were assessed radiographically, and functional outcome was determined with use of preoperative and postoperative American Orthopaedic Foot and Ankle Society ankle-hindfoot scores.All forty-five patients were available for follow-up at an average of 50.3 months.

The concentrations of 5-HT and 5-HIAA in tissue samples were determined with use of an electrochemical detector and the software CSW32 (DataApex, Soubêžnâ, Czech Republic).

Values of K, Rmin, and Rmax are determined with use of calibration kit (Invitrogen, Oregon).

Data are expressed as the means ± SD with n = 3. Statistical significances were determined with use of the unpaired Student's t test (P values < 0.05).

The concentration of CaCl2 required to increase resting free calcium in BAPTA internal was determined with use of the EGTAetc program, provided by EW McCleskey.

At 2, 4, 6, 8, 10 h and 1, 2, 3, 7, 14, 21 and 28 days a sample was removed from the media, the absorbance measured and the concentration was determined with use of the calibration curve.

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