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From previous literatures, we can find several mammalian SET domain genes that have been determined with expression analyses (e.g. Northern blot or RT-PCR assays), and those data are largely consistent with our WISH analyses of zebrafish SET domain genes (see below for examples).
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An association of FLA genes with tension wood in poplar has been determined with increased transcription and expression during tension wood production [ 41].
Based on these 21 matched 450 K CpG probes, correlations were determined with gene expression by using RNAseq expression data for all tumors (n =581) and separately for basal-like (n =102) and luminal A (n =321) tumors classified by PAM50.
Germ cell proliferation and apoptosis were determined with immunohistochemical expression of proliferating cell nuclear antigen (PCNA) and in situ DNA 3′-end labeling (terminal deoxy-nucleotidyl transferase-mediated digoxigenin-dUTP nick-end labeling, TUNEL) in the testes of infertile men with mixed patterns of MA, respectively.
Relative gene expression was determined with the formula fold induction=2−ΔΔCt, where ΔΔCt=(Ct GI (unknown sample)−Ct GI (reference sample))−(Ct GR (unknown sample)−Ct GR (reference sample)).
Fold changes were determined with the relative expression software tool [ 54].
LRP expression was determined with the mouse MAb LRP-56 (IgG2b) and BCRP expression was assessed using the MAb BXP-34 (Scheffer et al, 2000).
Gene expression measurements were determined with the Gene Expression Ct Difference (GED) formula [ 29].
IL-24, IL-20, and TNF-α mRNA expression was determined with a Taqman 20X Assays-On-Demand (FAM-labelled MGB-probes) gene expression assay mix (assay ID: IL-24: Hs01114274_m1; IL-20: Hs00218888_m1; TNFα: Hs00174128_m1).
U6 or GAPDH was used as the internal control, and the relative level of miR-363 or MCL-1 expression was determined with the 2−ΔΔCT method to calculate the fold change of the RNA expression [ 12].
Relative expression levels were determined with reverse-transcriptase quantitative PCR (RT qPCR) and transgene expression was found to vary by less than 8-fold between lines.
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