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The cutoffs of the polyclonal antibody based ELISA have been determined previously, with specificities of 96%and99%9% for fecal and urine specimens, respectively (11).
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We confirmed the stoichiometries of phi29 components that were determined previously with microscopic approaches.
The working concentrations of the inhibitors used were determined previously with dose response assays (not shown).
Specificity of our anti-AQP4 antibody was determined previously with the use of AQP4 knockout mice (Binder et al., 2006).
However, even though partial volume corrections (PVCs) were not performed CBF has been determined previously both with and without PVC across several dementia groups [ 36].
The affinities of this panel of five scFv clones have been determined previously [ 12- 14], with a range of equilibrium dissociation constants (Kd) ranging from a lowest of 320 nM to the highest at 0.013 nM.
The threshold OD was determined previously by testing sera from patients with TSS caused by TSST-1.
AOPPs have been determined as described previously with modifications [29,30].
However, atomic-level interactions had previously been determined only with antibodies from a single donor, thus making commonalities in recognition uncertain.
The pre-exponential factor S0 and the heat of dissolution Hs have been determined and compared with previously reported values for other networks and linear polymers.
The HCV genotypes for these samples had previously been determined with the Versant™ HCV genotype Assay LiPA (version I; Siemens Medical Solutions, Diagnostics Division, Fernwald, Germany).
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