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Blood methylation status in these women had been determined previously using the HumanMethylation27 BeadChip as part of a case cohort study of methylation and breast cancer.
Rates of reaction were measured with ppp*G 1 A73- and ppp*G 1 C73-tRNAHis [for which rates had been determined ppp*G 1 C73-tRNAHisP-labeled tRNA (Table 1)] as described in Materials and Methods.
The structures of only three glycosyl-enzyme intermediates in the GH13 family have been determined previously using either 5-fluoro substrate analogues, substrate analogues that were unable to act as acceptors, or activated donor substrates with an enzyme lacking its acid/base residue.
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The stress-induced axial stain along the fiber length was determined previously using fluorescence spectroscopy.
The optimum irradiation conditions were determined previously using caged fluorescein dextran.
Certain secondary structural features in the map were discernible and allowed approximate placement of a CTX molecule, whose structure was determined previously using NMR.
The solution NMR structure of RPA32C in complex with a UNG2 peptide was determined previously using a 99-residue RPA32172 270 construct.
MICs of 9EA-FC-B and ampicillin against MRSA ATCC 43300 which was 3.00 mg/mL and 0.05 mg/mL respectively were determined previously using broth microdilution assay conducted at 35°C [ 15].
These have to be determined previously, preferably using the iterative procedure introduced in this paper.
Specificity of our anti-AQP4 antibody was determined previously with the use of AQP4 knockout mice (Binder et al., 2006).
Temperature profile has previously been determined analytically using the boundary layer and lubrication theory techniques.
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