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Our detection strategy depends on the use of PCR targets in the L gene of a paramyxovirus and has been determined by sequence availability.
DOX-treated patients for which p53 status had been determined by sequence analysis [ 2] were included in our analysis (102 tumor samples, including 8 normal-like breast samples, one unclassified tumor, and 37 before and after pairs, representing 69 patients in total).
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The percentage of nucleotide (nt) and amino acid (aa) identity between mecD and other mec genes was determined by sequence alignment with Clustral OMEGA [ http://www.ebi.ac.uk/Tools/msa/clustalo/].ac.uk/Tools/msa/clustalo/
The relationships between mRNAs and proteins are determined by sequence similarity searches.
In drug design, for example, the function of a protein is determined by sequence comparison and 3D modelling programs.
The insertion sites were determined by sequence analysis.
The transmembrane regions are determined by TMHMM [17] and the glutamate binding regions are determined by sequence alignment.
The HLA type was determined by sequence specific primer (SSP -PCR in the Guangzhou Organ TranSSP -PCRnd Tinsue theinGuangzhou
The Mamu-DRB allele of individual macaques was determined by sequence analysis using the Genbank data base [15], [20].
The replication origin was determined by sequence similarity to the origin of Shewanella oneidensis and GC nucleotide skew analysis [45].
Furthermore, genes potentially involved in the effect of one stimulatory metagenomic clone were determined by sequence analysis associated to mutagenesis by transposition.
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