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The health status of mussels has been determined applying an expert system for classification able to rank the stress syndrome evolution by integrating the results from a battery of biomarkers [ 38].

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Melting points of PCL in blends were determined applying a step-wise annealing procedure.

Sex ratio of the offspring was determined applying a technique of molecular sexing described by Katsushima et al. [23].

As published elsewhere [ 17], absolute levels of GLA and DGLA in human plasma were determined applying a gas chromatographic method with mass selective detection.

A final score was determined applying a weighting factor to each estimate, following a formula: H-score=(low %) × 1+ medium %) × 2+(high %) × 3. The results ranged from 0 to 300, and the median values were considered as high expression cutoffs.

Protein concentration was determined applying the Bradford assay (Bio-Rad).

Irreversible losses are determined by applying an exergy balance on each of the systems investigated.

The trap frequencies are determined by applying an AC signal to one of the compensation electrodes and scanning the applied frequency over the frequency range supposed to contain the trap frequencies while monitoring the the rate of fluorescence photons.

The areas of the different chromatin compartments were determined by applying an intensity threshold on low pass filtered images.

Statistically significant responses were determined by applying an initial Z-value (i.e., Gaussianised-t) cut-off as a cluster creation threshold, and assessing the size of contiguous clusters of voxels against Gaussian random field theory, correcting for multiple comparisons across the search volume to p≤.05, corrected.

The expression of SLC22A1 was determined by applying an immunohistochemical score, as described in the Materials and methods.

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