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Exosomes have been detected in cell culture supernatants as well as body fluids, and are composed of a lipid bilayer.
Low BRMS1 expression levels have been detected in cell lines [ 8, 9, 11- 13, 22] and tissue specimens [ 11, 13, 19] from various human cancers.
Colorectal tumours often show altered RC function and mtDNA mutations have been detected in cell lines derived from these tumours (Polyak et al., 1998).
Expression of c-Met and/or HGF has been detected in cell lines established from pediatric tumors including rhabdomyosarcoma, osteogenic sarcoma, and neuroblastoma [ 12, 18, 19].
Tobacco smoke-induced methylation and resulting loss of gene expression has been detected in cell lines derived from cigarette smoke- or tobacco carcinogen-induced mouse lung tumors that showed reduced expression of death associated protein (DAP) kinase.
Nonetheless, siRNAs produced from LINE-1 retrotransposons have been detected in cell culture (Yang and Kazazian, 2006), and high-copy transfected plasmids expressing a sense-antisense RNA pair do produce detectable siRNA in HEK293 cells (Nejepinska et al., 2012b).
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Catalases have been detected in cell-free extracts or in solution after permeabilizing yeasts of H. capsulatum, suggesting that these were secreted enzymes [53].
Since miRNAs have been detected in cell-free body fluids [17] [20], we also performed direct isolation of total RNAs from culture medium without procedure for isolating exosomes.
Interestingly, miRNAs have been detected in cell-free human plasma preparations.
In limited numbers, miRNAs have been detected in cell-free human plasma preparations.
The smoking effect has even been detected in cell-free DNA present in plasma.
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