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As far as we know, differences in expression of histone genes across tissues and treatments have not previously been detected by microarray analysis, and this result is only detectable using the WT approach.
A more precise measurement of transcript abundance of these genes was performed by qRT-PCR, which revealed that differential expression was significantly greater than had been detected by microarray: the range of fold difference between ALL and AML by gene expression array was 1.0 2.8 and by qRT-PCR 2.3 45.6 (Figure 3B).
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Amplifications of large chromosomal regions have been detected by microarray-based comparative genomic hybridization in breast tumors and breast cancer cell lines.
The freshly isolated resting mouse peritoneal macrophages were stimulated with different cytokines in vitro, the expression of cytokines and chemokines were detected by microarray, real-time PCR, ELISA and multiple colors flow cytometry.
In this study, ssDNA intermediates resulting from the processing of meiotic DSBs were detected by microarray hybridization.
Because undifferentiated spermatogonia were present at a low concentration, these genes could not be detected by microarray analysis.
DNA microarray analysis revealed that GR5 is highly divergent; its genes were not sufficiently similar to be detected by microarray hybridization in any other Cronobacter strains.
Genes encoding leukocidins and exfoliative toxins as well as protease genes splA, splB and splE cannot be detected by microarray hybridisation.
However, it has to be taken into account that all these genome wide studies measure only the mature mRNA that has not been degraded and can be detected by microarray or RNAseq technology.
Overall, these results were consistent with hypothesis 2, which claimed that the host gene expression of animals with MCF was affected in a manner that could be detected by microarray analysis and that could explain the disease phenotype without all dysregulated cells being infected.
Taken as a whole, these results were consistent with one of our hypotheses, which claimed that the host gene expression of animals with MCF was affected in a manner that could be detected by microarray analysis and that could explain the disease without all dysregulated cells being infected.
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