Sentence examples for been detected by adding from inspiring English sources

These chemical compounds had ironically been detected by adding water brought all the way from Earth.

After a final wash, the membrane signals were detected by adding chemiluminescence detection reagent provided with the kit and the membrane was exposed to X-ray film for different time intervals.

The horseradish peroxidase-conjugated secondary antibody (Pierce Chemical) was used and the signals were detected by adding the enhanced chemiluminescence western blotting detection reagents (Amersham Biosciences, Piscataway, NJ, USA).

Islanding is detected by adding the WSE of each phase to obtain WSE index (WSEI).

This can be detected by adding scavengers in the solution and will be studied in our further experiment.

The surviving cells were detected by adding 20 μL MTT (5 mg/mL) and incubating for 4 h.

The bound phages were detected by adding 50 μl HRP-conjugated anti-M13 antibody (Pharmacia, diluted 5000 times with PBS) followed by an incubation of 1 h.

Bound antibodies were detected by adding rabbit anti-human IgG peroxidase conjugate for 30 min, followed by staining with tetramethylbenzidine for 15 min.

After rinsing with Ca2+ buffer, the binding between AnnV-Bt and the target was detected by adding the ABC Standard Peroxidase Staining Kit (Thermo Fisher Scientific) (200 μL) for more than (over) one hour at room temperature.

Antibacterial activity was detected by adding 0.5% TTC (Merck) aqueous solution and antifungal activity by adding 10 μl of a (3- 4,5-dimethylthiazol-2-yl -2,5-diphenyltetrazolium bromide (MTT) solution [5 mgml-1 MTT in phosphate buffered saline (PBS), pH 7.4].

Various dose of PGRN was coated to ELISA plate, biotinylated DR3, TNFR2 (serving as a positive control) or BSA (serving as a negative control) was then added to each well, bound protein was detected by adding avidin-HRP to each well and the absorbance was measured at OD 450 nm.