Sentence examples for been detached using cell from inspiring English sources

Cells that had been detached using Cell Dissociation Buffer (Gibco/BRL 13150-016) were allowed to attach to either collagen I, collagen IV, or fibronectin for 10 min before washing to remove nonadherent cells followed by fixation of adherent cells.

Cell spreading was determined by re-plating cells that had been detached using Cell Dissociation Buffer on either Collagen I or Fibronectin coated dishes in 1% FCS (BD BioCoat Variety Pack 354431) and recording their behaviour by time-lapse microscopy.

Following this, cells were washed twice with cold phosphate buffer saline (PBS) and were detached using cell scrapers.

Fresh PBS (10 ml) was added and the cells were detached using cell scrapers.

Subconfluent cells from a 6-cm dish were detached using cell dissociation buffer (Gibco/BRL 13150-016) before centrifugation at 250  g.

Cells were detached using cell dissociation solution (CDS) and lysed by freezing at −80 °C after adding 0.1 % Dodecyl-D-β Maltosid with proteinase inhibitor.

Endothelial cells were detached using a cell scraper (Greiner).

The cells were detached using a cell scraper.

The old DMEM was aspirated off and 3 mL of fresh DMEM was placed in each flask and cells were detached using a cell scraper.

The supernatant was discarded, the cells were detached using a cell scraper and resuspended in 2 ml PBS (138 mM NaCl, 8.1 mM Na2HPO4, 1.47 mM KH2PO4, 2.67 mM KCl; Invitrogen, cat. no. 21600-069, lot. no. 1255481) per plate.

The PBMCs were detached using a cell scraper.