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We collected 120 abandoned branches that had been detached by O. albomarginata chamela, in a tropical dry forest, in Jalisco, Mexico.
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Cells were detached by Macrophage Detachment Solution DXF (PromoCell) following the manufacturer's protocol.
U937 macrophage-like cells were detached by Macrophage Detachment Solution DXF (PromoCell) following manufacturer's protocol.
"You mean my son is detached by nature," he said.
The adherent primary hepatocytes were detached by scraping and resuspended in phosphate-buffered saline (PBS).
AT-cells were detached by vigorous pipetting in PBS/5% FCS and harvested.
"It just so happens that my cockpit is detached by 400 or 500 miles," he said.
However, C. parvum oocysts captured on the glass slide were detached by repeating washing steps.
The gene modified cells could be detached by thermo-triggered switchable surface hydrophilicity of PMEO2MA in hydrogel.
Cells (2×105 cells after counting) were detached by Accutase and spun down at 600 ×3 g for 3 min and then washed with PBS.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com