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However, most applications of HT technology to-date have been designed to sequence a large number of fragments from relatively few individuals.
The present study has been designed to sequence and analyze extra-pulmonary isolates to identify genomic patterns and features of M. tuberculosis isolated from patients with extra-pulmonary tuberculosis.
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Primers were designed to sequence the lukSF-PV genes.
Primers were designed to sequence regions of the genes with minimal nucleotide differences between the two species.
Our microarray was designed to sequence all exons and 8 base pairs (bp) of flanking intronic regions from the splice junctions of CHRNA1, CHRNB1, CHRND, RAPSN, COLQ, CHAT, and MUSK (Table 1).
Two primers were designed to sequence of 30 nucleotides within the PCR amplicons generated from plasmids pXO1 and pXO2, respectively.
BAC clones likely to contain the orthologous gene were identified based on synteny and primers were designed to sequence the genes directly on purified BAC DNA.
The instrument is based on Combinatorial Probe-Anchor Ligation chemistry [ 182] and is designed to sequence up to 10,000 genomes per year with a 50x coverage.
In order to detect the breakpoints with accuracy, two internal primer pairs were designed to sequence introns 9 and 10 across the deletion (Table 3).
Three sets of primers were designed to sequence from the promoter region to exon 3 of the Atlantic salmon IGF1 gene (Table 1).
Primers were designed to sequences for the desired genes using Primer Express 2.0 software (Applied Biosystems).
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