The peptidyl loop included one lysine residue (abbreviated as the K-loop), and was designed and inserted into two different regions of the protein bacterial alkaline phosphatase (BAP).
Within the scope of the design and qualification of a helium-cooled lithium lead (HCLL) blanket module for DEMO, test blanket modules (TBM), have to be designed and inserted in ITER for testing.
Based on a bioinformatics approach, three human CD19-derived sequences were designed and inserted into a self-inactivated lentiviral vector backbone upstream of the GFP gene: S.CD19 (230 bp), M.CD19 (464 bp), and L.CD19 (1274 bp).
For compensating non-uniformity introduced by the particle flux profile and the radially dependent track speed on the turntable, baffle elements have to be designed and inserted beneath the substrates.
To suppress BMI-1 expression in osteosarcoma cells, short harpin RNA (shRNA) targeting BMI-1 gene was designed and inserted into the recombinant lentivirus plasmid.
pCAG-EGFP/RFP-miR-Pgrn1 and 9 target the mouse progranulin gene and they were constructed as follows: miRNAs were designed and inserted into the pCAG-EGFP/RFP-miRNAint backbone as described previously [15].
A synthetic oligonucleotide containing the vicrostatin (VCN) and the RGD peptide sequence was designed and inserted via cassette mutagenesis into the pET25b cloning vector (Table 1).
Four short hairpin RNA (shRNA) sequences targeting the β1-integrin gene and one negative control sequence were designed, and inserted into the lentivirus vector GV115 containing a green fluorescent protein (GFP) reporter.
This working electrode was designed and fabricated by inserting a PEDOT PSS film between a fluorine-doped tin oxide (FTO) glass substrate and a layer of nanocrystalline TiO2 particles (P-25).
To release the residual stress, sandwich structure was designed and the metal interlayer was inserted between the diamond films and the substrate.
