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Cadmium was chosen as a model toxicant because the effects of exposure have been described using several feeding assays [17], [25], [26].

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Structural extension is not limited to proteins, and recently several approaches have been described using RNA secondary structures as templates, these include T-Lara (Bauer et al., 2005), MARNA (Siebert and Backofen, 2005) and R-Coffee (Wilm et al., 2008).

Several models have been described using immortalized endothelial cell lines such as mouse brain endothelial cells bEnd.3 [29], rat brain endothelial cells RBE4 [30,31], and hCMEC/D3 human endothelial cells [32].

Typically, model parameters are fitted against a single measured moment of the cluster distribution which can usually be equivalently described using several variations of the set of parameters underlying the relevant aggregation, breakage and restructuring kernels.

In other cases, an EC entry may give only a general description of the overall reaction whereas the different steps of this chemical transformation may be more precisely described using several reaction steps.

The presence of this partially unfolded form in the three PsTRXs do not agree with the acidic denaturation observed in TRX of E. coli, where the secondary and tertiary structure of the protein does not change from pH 3.0 to 7.0 [62]; notwithstanding these facts, kinetic intermediates during the folding of E. coli TRX have been described by using several techniques [62]-[64] [62]-[64]

OV elongation has been described in several vertebrates using fixed tissue analysis, but not its underlying mechanism (Hilfer, 1983; Schmitt and Dowling, 1994; Schook, 1980b; Silver, 1981).

Another method for the determination of labial surface curvature, which has been described by several authors, uses a device that engages in a standard edgewise bracket bonded on the tooth surface [19],[19].

Extraction of DNA from wood followed by successful amplification of selected DNA regions has been described for several tree species using modified CTAB protocols (Ohyama et al. 2001; Reynolds and Williams 2004; Rogers and Kaya 2006).

Tumoural growth reduction in nude xenograft models has been described in several studies either using an shRNAi model (Smith et al, 2006) or through the inhibition of EWS FLI1 targets such as IGF-1/IGF-1R (Scotlandi et al, 1998), VEGF (Guan et al, 2005), NR0B1/DAX1 (Kinsey et al, 2006), and cholecystokinin (Carrillo et al, 2007).

Ultrasound use has been described in several helicopter EMS programs in the USA (in Portland, Ohio, and Minnesota).

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