An allosteric inhibition effect is demonstrated in specific regions of the protein.
Patterns of gene expression are demonstrated in specific tissues that may be masked in analysis of heterogenous samples.
Finally, while the mammalian genome contains two orthologous Gcm genes, their function has been demonstrated in vertebrate-specific tissues, placenta, and parathyroid glands, begging questions on the evolutionary conservation of the Gcm cascade in higher organisms.
Accordingly, the contribution of several of these β3-expressing cell populations to tumor growth and metastatic progression has been demonstrated in studies using specific inhibitors and/or genetic ablation of β3 receptors in animal models [ 10- 15].
Reduced glucose tolerance has been demonstrated in a liver-specific GCK mouse knockout (15).
A tissue-specific expression pattern of isomiRs during development has been demonstrated in Drosophila, suggesting a specific role of isomiRs in gene regulatory networks [ 31].
Rather than the "positive feedback" system of LA513, use of a late-acting promoter in a more conventional bipartite expression system might perhaps give more reliable control over the time of expression of an effector molecule and hence the lethal phase; this has previously been demonstrated in Drosophila for female-specific promoters and for embryo-specific promoters [ 27, 39, 40].
This has also been demonstrated in vivo, where the specific ETB antagonist (BQ788) was shown to significantly slow melanoma tumour growth in nude mice (Lahav et al, 1999).
Declines in physical and mental health due to critical incident stress in disaster aid workers or rescue workers has been demonstrated in previous research, but specific, adequate measures to counter critical incident stress have not been developed.
