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More recently, seed zones have been delineated using a genecological approach for several nontree native plants used in restoration, including Elymus glaucus (Erickson et al. 2004; Kitzmiller 2009), Bromus carinatus (Kitzmiller 2009; Johnson et al. 2010a, b), Bromus orcuttianus (Kitzmiller 2009), Festuca roemeri (Wilson et al. 2008), and Holodiscus discolor (Horning et al. 2010).
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Forest degradation due to logging was delineated using a 1 km buffer around identified logging roads.
First, geographical sub-areas were delineated using a parent lithology data layer.
For the data set of centre B, Tvol was delineated using a cut-off of the TBR > 1.6 based on a previous biopsy controlled study [8].
An averaged image of the last three frames of the scan was produced and lesions were delineated using a 50% threshold of the peak standardised uptake value (SUVpeak).
SEPs ranging from 0.0 to 0.78 were assigned to 176 unique ecoregions that were delineated using a combination of three mapped climate variables (Table 1) and existing forest cover (see Additional file 1: Appendix 1 for detailed methods).
The background ROI was placed in the contralateral hemisphere to the tumour in an area of normal-appearing brain tissue including white and grey matter, identically for centres A and B. For the data set of centre A, the tumour volume (Tvol) was delineated using a cut-off of the TBR > 1.8 which is based on the clinical experience in that centre [16, 20].
Fire regions were delineated using a length of frost-free season (a proxy for fire-season length), growing season precipitation (a proxy for fuel-moisture availability), mean temperature of warmest month (a proxy for fire-season temperatures), and current forest cover (to capture fine-scale topographic influences on fire behavior not resolved in the other climate variables).
The striatum was delineated using a 4× objective, in accordance with a rat brain atlas [65].
Briefly, inserts were amplified using the M13 forward primer, sequencing reactions were performed using BigDye Terminator v3.1 (Applied Biosystems), and sequences were delineated using a PRISM™ 3730xl DNA Analyzer (Applied Biosystems).
Finally, a gene regulatory network was delineated using a novel Bayesian-based algorithm that can incorporate information from diverse datasets.
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