Sentence examples for been deleted using a from inspiring English sources

A second construct also contained cDNA coding for amino acids −36 to +139 but was mutated in the NLS: residues 88 91 and 102 106 had been deleted using a Transformer Site-Directed Mutagenesis Kit.

Genes were deleted using a markerless gene-deletion system as described previously (Sun et al. 2013).

In addition, items that still misfitted after the collapsing, were deleted using a step-wise top-down deletion strategy until the remaining items fit the model [ 83].

To construct a single-deletion "starter" strain library, the TF-encoding open reading frames were deleted using a PCR-based gene replacement strategy conferring uracil prototrophy or kanamycin resistance.

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In this study, the Fas2 genes of the human pathogen Candida parapsilosis were deleted using a modified SAT1 flipper technique.

During mammary gland development, defects were similar in Pofut1 and Rbpj mutants, when genes were deleted using a MMTV-Cre driver line [34].

The synthetic gene coding for HIVCONSV (GeneArt) was subcloned into plasmid pTH [45] and the codons coding for the H epitope were deleted using a PCR assembly to generate pTH.HIVCONSVdH.

Potential binding sites of the response regulator MbrC were deleted using a PCR-driven overlap extension method [ 120].

The slarp gene (PF3D7_1147000) in P. falciparum WT parasites (NF54wcb) was deleted using a modified construct based on plasmid pHHT-FRT- GFP -Pf52 (van Schaijk et al., 2010).

The b9 gene (PF3D7_0317100) of PfΔ slarp-b P. falciparum parasites was deleted using a modified construct based on plasmid pHHT-FRT- GFP -Pf52 (van Schaijk et al., 2010).