Sentence examples for been deleted by using from inspiring English sources
To test whether the suppression of the tpp1− phenotype is due specifically to the loss of stpA function, we constructed a double mutant strain, tpp1flox− /stpA−, in which the entire stpA open reading frame had been deleted by using homologous recombination.
Afterwards argG was deleted by using pK19mobsacB∆argG to obtain CIT0.
Some form features were deleted by using an attribute reduction algorithm based on Rough Sets Theory (RS T.
The asdA gene was deleted by using the described suicide vector technology.
In C. acetobutylicum, spo0A was deleted by using a replicative plasmid pETSPO capable of integrating into the chromosome through two rounds of crossover selection [36].
The gfa1 gene of the fission yeast Schizosaccharomyces pombe was deleted by KanMX6-mediated gene disruption and the Cre-loxP marker removal system, and the glmS gene of Escherichia coli was deleted by using λ-Red mediated recombinase system.
For the evaluation of its industrial applicability we tested this system in heterologous and homogenous SED1-disruptants of sake yeast, a diploid S. cerevisiae strain, in which either the SED1 ORF or the complete gene including the promoter was deleted by use of the high-efficiency loss of heterozygosity method.
Comey has previously testified to Congress that thousands of the uncovered e-mails could potentially have been considered work-related, but that they had likely been deleted by Clinton in the normal use of her account, and therefore would not have been among the e-mails and documents her lawyers reviewed.
To generate the cytosol-targeting ELuc expression vector pCMV-Flag::ELuc (cyto), the ELuc cDNA in which the peroxisome-targeting signal (PTS; Ser-Lys-Lue) at the extreme C-terminus was deleted by PCR using pBlue-ELuc as the template, and the product was ligated to the EcoRV/XhoI site of pCMV-Tag2B.
The miR-205-5p miR-205-5p miR-205-5p (5 bpredicted was deletarget PCR usite the following primers: Her2MutFw 5′- gccctgatgtgtcctcagggagcaggcc-3′ and Her2MutRev 5′-tgatgccagcagaagtcaggcctgctcc-3′ with QuikChange Lightning Site-Directed Mutagenesis kit (Agilent Technologies, Santa Clara, CA, USA).
saeRS was deleted by allelic exchange using the pMAD vector, as described by Arnaud et al [36].
