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Since CymR regulates expression from the p- cym and p- cmt operons, both sequences have been defined as putative operator sequences (CuO).
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Proteins that showed at least a 2-fold difference in ratio between the moderate and severe injury models were defined as putative biomarkers of SCI severity.
All SNP containing sequences that fulfilled these criteria were defined as putative candidate genes.
Among 1,286 LRR genes identified in soybean, 319 genes were defined as putative NBS-LRR genes.
The 3FTx toxin gene sequences that were derived from the same BAC clone were defined as putative tandem duplicates.
Based on the positions of representative TSSs, sequences from -1000 to +200 were defined as putative promoter sequences.
The Arabidopsis genes homologous to a given barley gene with the lowest e-value were defined as putative Arabidopsis orthologs.
We defined β hemoglobin genes exhibiting three hallmarks of a lack of the Bohr effect were defined as putative non-Bohr β hemoglobin genes.
A pair of best hit was defined as putative orthologs when the pair was aligned over 300 bp or more (e < 10-20).
If, after this hands-on annotation, predicted proteins still did not meet the above criteria, the sequences were defined as putative pseudogenes.
Genes that satisfied BLASTP alignment threshold (35% sequence identity over 80% length of the smallest protein) or bidirectional best hit criteria, were defined as putative orthologs.
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CEO of Professional Science Editing for Scientists @ prosciediting.com