If affected cows had been culled at an age comparable to the age of non-affected cows, they would have produced significantly less milk (Table 4).
Body weights and clinical signs of mice were recorded daily after challenge and, where necessary, mice were culled at a predetermined humane endpoint under guidelines approved by the University of Melbourne Animal Ethics Committee.
The phase of peak expression of PER2::LUC was only assessed for cultures collected from mice housed in DD as these individuals were culled at a wide range of times throughout the circadian cycle.
However, most of them are culled at a juvenile age due to the low growth performance, explaining the lack of FH2-homozygous animalsnimanimals
However, in study 1 cattle were culled at a predetermined time point post challenge or, in both studies, at a predetermined end point when the development of BSE was not expected in surviving animals.
One such example could be based on region-specific measures of the effect of homozygosity in order to generate the expected reduction in performance due to inbreeding and/or the probability of being culled at a given parity.
Controls which were culled at a later point in the epidemic were included if the animals on the premises were slaughtered more than 14 days after the source was slaughtered.
All foster cows had been culled at our first visit to the farm, and were not sampled.
Thirteen out of the 20 milk shedders had been culled at the end of the study, including the five cows that were considered persistently milk shedders.
Eight animals per group were culled at day 55 following an overnight fast to represent an age known to precede the development of increased adiposity and altered insulin sensitivity in UN offspring.
