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The catalytic domain of human recombinant MMP-12 has been crystallized in complex with the broad-specificity inhibitor batimastat (BB-94).
To investigate the substrate specificity, Mm-dAK has been crystallized in complex with dATP and dCTP, as well as the products dCMP and dCDP.
In the past three years, several polynucleotide ligases have been crystallized in complex with nucleic acid, providing the introductory views of ligase enzymes engaging their substrates.
This was demonstrated by a hybrid inhibitor that interacts with both the substrate and cofactor binding sites and a recently designed inhibitor 3- 3′,17′β-dihydroxyestra-1′,3′,5′(10′)-trien-16′β-methyl) benzamide which has been crystallized in complex with 17β-HSD1.
Recently, the core domain of human DDX3 has been crystallized in complex with AMP [18].
Oligomannose-3 has been crystallized in complex with the FimH RBD.
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The vancomycin-related antibiotics balhimycin and degluco-balhimycin have been crystallized in complexes with di-, tri- and pentapeptides that emulate bacterial cell-wall precursors, and four structures determined at atomic resolution (<1 Å).
The two subunits for which there were no inter-subunit cross-links have been crystallized in complexes with other Mediator subunits: Med6 as part of the Head module (Robinson et al., 2012), and Med31 in a complex with the N-terminal region of Med7 (Koschubs et al., 2009).
To study the molecular mechanism underlying Bbp-ligand recognition, Bbp273−598 was crystallized in complex with the ligand Fg α561−575.
The single-chain variable fragment of each antibody was crystallized in complex with the CD81 large extracellular loop in order to guide affinity maturation of two distinct antibodies by phage display.
To further understand the interaction of hK5 with substrates and metal ions, active recombinant hK5 was crystallized in complex with the tripeptidyl aldehyde inhibitor leupeptin, and structures at 2.3 Å resolution were obtained with and without Zn2+.
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