Sentence examples for been created by sequencing from inspiring English sources

Due to the high abundance of SKL > sequences in the underlying dataset and the close codon similarity between Ser (AG[TC]) and Arg (AG[GA]), the two RKL > positive example sequences could have been created by sequencing errors in ESTs and caused the false prediction of RKL > and RKM > sequences as peroxisomal.

Inducible promoter systems, such as T7/lac and Ptrc, have been created by fusing repressor sequences to promoter sites.

Expressed sequence tags (ESTs), which are created by partially sequencing randomly isolated gene transcripts and converting them into cDNAs [ 1], provide a valuable information source for investigating a wide variety of genetic characteristics of a species.

ESTs are created by partially sequencing randomly isolated gene transcripts and they play an important role in gene discovery.

It is important to note that the ESTs used were created by capturing sequences from the 3' UTR and sequencing from either the 3' or 5' end.

Non-redundant (NR) transcript sets were created by clustering sequences with cd-hit-est version 4.6 [ 46] with sequence identity threshold set to 1.0 and retaining cluster representatives (the longest sequences).

When using LDA-transformed concatenated features, a new equally large set of exemplars was created by selecting sequences of 29 consecutive frames, using the same procedures as for selecting single-frame exemplars.

A dataset was created by clustering sequences with a distance threshold of 97%.

The expression vector was created by inserting sequence corresponding to the (His 6 and XP tags of the Invitrogen pcDNA3.1/His C vector (Cat. no. V385-20) inthethe Kpn I site of the pCMV-Sport-βgal vector, following removal of the βgal ORF.

Sequencing libraries were created by ligating adaptor sequences to the pooled DNA using the Ion Plus Fragment Library Kit (#4471252, Life Technologies).

Details of the sequence homologues found are provided in Additional file 6. Consensus zebra finch chicken sequences were created by aligning homologous sequences using MEGA3 software [ 44] and replacing mismatching bases and gaps with the code "n" to represent an unknown base.