Gene expression is modulated by trans-factors and upstream and downstream non-coding sequences and therefore the molecular evolution of genic sequences need not be coupled to expression evolution.
Results This study describes the generation of the first Drosophila spliceosensor model – transgenic flies in which the expression of human disease-linked splicing variants expressed from minigenes is coupled to the expression of a reporter – and their use in the in vivo identification of splicing modulators for DM1.
This heating was coupled to transgene expression by using a heat shock inducible promoter.
In some organisms, swarming is controlled by quorum sensing, and in others, swarming has been shown to be coupled to increased expression of important virulence factors.
The distribution of proteins involved in the recirculation of divalent metal ions leads us also to believe that ferroportin is coupled to the expression of DMT1.
Each of these events is initiated by distinct signals that are coupled to the expression of specific cell surface proteins by corresponding signaling pathways (for reviews, see [ 13, 14]).
Stratification is coupled to the expression of K14 in the epidermal basal layer from the 13th week, in hair follicles and the basal layer of the sebaceous glands starting from the 16th week of development [ 2].
To this end, we have generated and validated transgenic flies that contain a luciferase-reporter-based system that is coupled to the expression of MBNL1-reliant splicing (spliceosensor flies), to assess events that are deregulated in DM1 patients in a relevant disease tissue.
Mice engineered to lack expression of both FoxA2 and FoxA3 in their chondrocytes display defects in chondrocyte hypertrophy, alkaline phosphatase expression, and mineralization in their sternebrae and, in addition, exhibit postnatal dwarfism that is coupled to significantly decreased expression of both collagen X and MMP13 in their growth plates.
The S-allele is coupled to reduced gene expression, leading to lower densities of the serotonin transporter [ 37, 38].
