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The library produced with the double-fragmentation method resulted in much more unique reads (1.93 M unique reads from 3.4 M mapped reads) as compared to the sample processed without secondary fragmentation (0.89 M unique reads from 3.4 M mapped reads), for this purpose when two or more reads have the same starting position on the same strand they were counted as single unique read.
* Just to be clear on how sessions are recorded by 7Park: if a user opens Snapchat but then gets a text or navigates away to another app, when they return it's counted as a new, unique session.
Every drug was counted as one with its unique ATC code regardless of the dosage or number of pills for the individual patient.
Unique reads were counted as a whole count, whereas multiple reads were counted proportionally for each location they were mapped to (up to five sites) when none of the adjacent unique reads appeared.
The occurrences of each unique sequence reads were counted as sequence tags (the number of reads for each tag reflects relative expression level) and only small RNA sequences of 18 to 30 nt were retained for further analysis.
After removing the adaptor/acceptor sequences, filtering the low quality tags and cleaning up the contamination formed by the adaptor-adaptor ligation, the occurrences of each unique sequence reads was counted as sequence tags.
Simultaneous bleeding events from unique organ systems were counted as individual events.
Clean reads of unique small RNA tags were counted as their expression abundances.
Such sex chromosome constitutions are unique among Lepidoptera and should be counted as an additional factor potentially contributing to the speciation process in Leptidea butterflies.
Similarly, KAP0 and KAP1, the regulatory subunits of protein kinase A, were counted as a single hit because of a lack of unique peptides identified for each protein.
If a sequence was non-unique to a single protein, it was counted as corresponding to each of the matching proteins.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com