Sentence examples for been confirmed previously using from inspiring English sources

Cellular proliferation in primary samples has been confirmed previously using [H]-Tdr uptake with pHH3 expression correlating extremely well with the amount of proliferation [ 22].

The ability of benzyl-α-GalNAc to inhibit MUC1 O-glycosylation has been confirmed previously using lectin labelling in HT-29 and Capan-1 cells (Leteurtre et al, 2003).

The specificity of the antibody used has been confirmed previously (1).

This has been confirmed previously [ 16].

In both liver and thymus, a strong age-related increase in the expression of such immune-associated genes has previously been confirmed using RT-PCR [ 30, 31].

The specificity of the 20-kDa fragment and its correlation to Cx43 has previously been confirmed using various antibodies, siRNA-mediated knockdown, and Cx43/GJA1 knockout mice [ 7, 13].

To validate our method, we assayed DNA samples from 244 patients and two commercially available genomic DNA samples, the genotypes of which had previously been confirmed using the PCR-direct sequencing method.

Androgen regulation was confirmed using previously published gene expression data, which supported the weak downregulation of PRDX-3 in response to androgen treatment (Massie et al, 2011).

After isolates were first shown to be non-susceptible to cefoxitin, the presence of AmpC β-lactamases in E. coli, K. pneumoniae and Salmonella isolates was confirmed using previously described boronic acid methodology [ 19].

The genotype of gene-knockout strains was confirmed by using a previously reported protocol [ 14].

The finding that intact Syk was involved in the efficient translocation of p110δ to the leading edge was confirmed using a previously described HL-60 cell clone that downregulates Syk protein expression by stably producing a Syk specific short-interfering RNA (Syk-siRNA) [10].