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Paper prescriptions are small, light and mobile and can be annotated, for example, in the final safety check the pharmacist can tick each item on the paper as it has been confirmed correct, allowing them to be interrupted and regain their place without losing accuracy.
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Briefly, initial denaturation time of 15 min was followed by 45 cycles of denaturation at 94°C for 1 minute, and annealing/extension at 60°C for 1 min. The PCR amplicon was confirmed correct by Sanger sequencing and characteristic melting temperature.
The SjTGR entire open reading frame amplified by PCR with special primers was subcloned into the plasmid pVAX1 with T4 DNA ligase and was confirmed correct by restriction enzyme digestion and sequencing.
The four tested methods had the same specificity for ruling out endobronchial intubation (that is, confirming correct tracheal intubation).
Danon disease has been confirmed as the correct diagnosis in 4% of patients initially diagnosed as HCM [46].
The sequences were confirmed as correct by sequencing the inserts in both directions using standard T7 promoter and T7 terminator primers.
All five changes were confirmed as correct by Sanger sequencing.
Lines in which singletons were found were analyzed again until they were confirmed as correct.
Singletons, which occurred only once as polymorphisms among the sequenced materials, were analyzed until they were confirmed as correct.
1,853 predictions were confirmed as correct by Sequenom genotyping and 120 were discordant (Table 2), to a calling accuracy of 93.9%.
Embryonic stem (ES) cell clones were confirmed for correct homologous recombination of both the 5′ and 3′ arms through Southern blot (Fig. 1B) and PCR.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com