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Many studies investigating the role of MMP-9 in CRC have been conducted using cell lines or animal models.
Because of the difficulty in obtaining sufficient amounts of fresh osteoblasts, osteoclasts, and osteocytes, most gene expression studies have been conducted using cell cultures.
The majority of the published studies that entail the quantitative mass spectrometry-based analysis of ubiquitylation using anti-K-ε-GG antibodies have been conducted using cell lysate [ 16– 20].
The majority of these studies, however, have been conducted using cell culture systems with transposon delivery from transfected plasmid DNA and/or with selection for cells that have integrated transposons.
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To date, the majority of cell-based assays have been conducted using cells that grow as 2D monolayers on the surface of polymer or glass dishes.
Most of these studies were conducted using cell lines that may have acquired significant modifications of their epigenetic status both during transformation and during prolonged cell culture.
All experiments were conducted using cell culture populations and not purified clones.
Related research will be conducted using cell lines or animal models in future studies.
Further comparative analysis was conducted using cell wall and cell contents auto-fluorescence confocal microscopy by Z-stacking with high resolution for closer comparison of cell content fragments to 3D images.
A structure activity relationship study was conducted using cell-surface expression assay to quantify folding/trafficking efficiency of P23H rhodopsin.
The electrochemical incineration of bromoamine acid (BAA), an intermediate of anthraquinone dyes, was conducted using cells containing boron-doped diamond (BDD) anode and Pt cathode under galvanostatic conditions.
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