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Several studies have been conducted to sequence the AML exomes and the trend is increasing.
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Pure cultures of each bacterial morphotype were streaked out, and colony PCR was conducted to sequence the conserved region of the 16S rRNA gene to identify bacteria.
On the other hand, comparisons of upstream non-coding sequences have been conducted to investigate the regulatory sequences.
Typically, sequences have been deposited in a database and all-by-all sequence comparisons with BLAST are conducted to assemble sequence clusters based on similarity.
The low molecular weight plasma proteome and its biological relevance are not well defined; therefore, experiments were conducted to directly sequence and identify peptides observed in plasma and serum protein profiles.
Further studies should be conducted to get sequence information to confirm the real HEV infection rates and to determine the HEV genotypes.
In addition, MS/MS experiments were conducted to obtain sequence information on the copolymers and oligomeric additives, whereas ultrahigh mass resolution was performed to separate molecules that are close in mass.
A small-scale expressed sequence tags (EST) project was conducted to generate sequences to be used in identifying antigens from a proteomics study of S. haematobium antigens [ 29].
Illumina sequencing was conducted to generate short sequence reads of expressed sequences.
BLASTN searches against the Rfam database, NCBI GenBank database and miRBase were conducted to remove sequences representing the fragments of non-coding RNAs (rRNA, tRNA small nuclear sequences and small nucleolar RNA), exons, introns and miRNA.
Two cycles of polymerase chain reaction (PCR) were conducted to amplify sequences corresponding to conserved regions II through V of FDS and CDS.
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