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Eleventh hour activity, therefore, has been concentrated with a view to capturing a decisive balance of feminine support.
Dilute solutions of biological macromolecules such as proteins have been concentrated with good recoveries and high selectivity.
Fecal samples of at least 25 g were examined for the presence of Cryptosporidium oocysts using bright-field microscopy of the fecal material that had been concentrated with the formalin ethyl acetate sedimentation method and Sheather's sugar flotation technique [ 24].
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Before use, LDL was concentrated with Centricon 10 concentrators (Amicon, MA) to a final concentration of about 7 mg/mL protein.
Peptide fractions were concentrated with a vacuum concentrator (Eppendorf), and kept in low-binding tubes at -20°C until further analysis.
Prior to transducing cells, virus particles were concentrated with Vivaspin 20 concentrators (Sartorius Stedim Biotech GmbH, Goettingen, Germany) according to the manufacturer's instructions.
The supernatant was concentrated with a gas blowing concentrator.
Total RNA was concentrated with a DNA100 SpeedVac Concentrator (Savant) and reverse transcribed using the High-Capacity cDNA reverse transcription (RT) kit (Applied Biosystems Inc., Foster City, CA).
Pooled PrimPol was concentrated with the Vivaspin protein concentrator.
Pure Irga6 proteins were concentrated with Vivaspin 20 centrifugal concentrators (Sartorius).
The eluted antibody was concentrated with Amicon Ultracel-50 k concentrators (Millipore, Billerica, MA, USA) and dialysed against PBS.
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