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Table 2 compares the gene content of B. hypnoides cpDNA with that of other Ulvophyceae, Trebouxiophyceae, and Chlorophyceae (UTC) algal cpDNAs that have been completely sequenced to date.
Eleven of the thirteen (excluding BS436 and BS437) clinical strains have been completely sequenced to an average depth of coverage of >20-fold and have been demonstrated to be unique with respect to their genic content [29], [31].
Specifically, "full length" F-ORF-VD1s were found in all M. californianus VD1 (n = 3), in all but 3 (12/15) M. trossulus VD1 and in all but 8 (41/49) of the M. edulis and M. galloprovincialis VD1 that have been completely sequenced to date (Figure S2) [40], [45], [46], [55] [57]]–[55] [57]
For these reasons, genomes of only four phytoplasma strains have been completely sequenced to date.
Because of their compact size and the availability of conserved DNA probes, many chloroplast genomes have been mapped [ 14], and 121 have been completely sequenced to date.
Since no legume genome has been completely sequenced to date, the pipeline relies on EST data and is not able to discern between orthologous and paralogous origin of homologous sequences.
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The genome of a novel methanogenic archaeon, namely Methanobacterium congolense Buetzberg, originally isolated from a mesophilic biogas plant, was completely sequenced to analyze putative adaptive genome features conferring competitiveness of this isolate within the biogas reactor environment.
All constructs were completely sequenced to verify accuracy of promoters and coding regions.
The resulting construct (p3.1M- DLK1-HA) was completely sequenced to confirm its integrity.
The gna1 coding region was completely sequenced to ensure that only the desired mutations had been introduced.
The insert of the resulting plasmids was completely sequenced to confirm the mutation and discard the presence of undesired mutations.
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