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Demonstration of this process has been completed using real flue gas in a pilot plant located at Hazelwood power station in Victoria, Australia.
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RT-PCR was completed using a TaqMan® real-time method.
Amplifications were completed using a 7500 real-time PCR system (Applied Biosystems, Foster City, CA).
Analysis of BMSC Interleukin-6 (IL-6) expression was completed using ELISA and real-time PCR.
Base calling was completed using Illumina Sequence Control Software with Real Time Analysis.
Quantitative real-time polymerase chain reactions were completed using a Brilliant II SYBR Green QPCR Master Mix in an Mx3000P instrument (Stratagene, La Jolla, CA, USA).
The qPCR was completed using iQ SYBR Green Supermix (Biorad) and the iQ Multicolour Real-Time PCR Detection System thermocycler (Biorad) was used for detection.
Autonomous perception of critical phenomena and structures is completed using the coupled force and torque drilling transients, described above, in real-time.
Quantitative real-time polymerase chain reaction (qRT-PCR) amplification was completed using the ABI Prism 7900 Sequence Detection System (Applied Biosystems).
All computations were completed using STATA v10.0.
Six permits have been issued and one inspection has been completed in real-time using a mobile device.
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CEO of Professional Science Editing for Scientists @ prosciediting.com