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Prior to the present study, gene expression patterns in patients with constitutional trisomies have exclusively been compared with expression signatures derived from disomic control cells from unrelated healthy individuals [ 25- 31].
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For that reason, expression profiles of pluripotency associated factors in blastemal cells were compared with expression profiles in pluripotent embryonic reference cells.
In each case the adenoma expression levels were compared with expression levels in normal bowel mucosa taken from the same patient as previously described (Caldwell et al, 2004).
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