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Lane 5. Enzyme: exochitinase eluted from ion-exchange chromatography after been cleaved from a MBP/exochitinase.
SPP is an enzyme of the endoplasmic reticulum (ER) that catalyzes intramembrane proteolysis of signal peptides after they have been cleaved from a preprotein by a signal peptidase [34], [37].
SPP, the prototypic member of the human SPP family of aspartyl proteases catalyzes intramembrane proteolysis of some signal peptides after they have been cleaved from a preprotein and also of several viral preproteins [23] [25].
SPP catalyzes intramembrane proteolysis of some signal peptides after they have been cleaved from a preprotein.
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Specifically, 5 × 5 mm2 InSb chips, which were cleaved from a 2-in diameter InSb (100) wafer, were set on a 4-in diameter ceramic TiO2 target.
The gold wafers on which the NiCu was deposited were cleaved from a silicon wafer plated with 1,000 Å of gold over a 50 Å titanium adhesion layer (Platypus Technologies, LLC, Madison, WI, USA).
Exfoliation is a mechanical method, firstly used by Geim and Novoselov, in which single or few layers of graphene is cleaved from a stack of hundreds of thousands of graphene layers.
The working electrode substrates were cleaved from a silicon wafer plated with 1000 Å of gold over a 50 Å titanium adhesion layer (Platypus Technologies, LLC, Madison, WI, USA).
It was cleaved from a glutathione-affinity column using PreScission protease (Amersham Biosciences), followed by gel-filtration chromatography.
While the nature of the labelled molecule(s) present in the tissues was not identified in these studies Nöhle and Schauer proposed a model where Sia is cleaved from a glycoconjugate followed by Npl catalysed cleavage to ManNAc and pyruvate [19].
TNFα is cleaved from a transmembrane bound precursor, from where it binds a TNFα receptor.
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