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Spray areas that have not been cleaned previously with an enzymatic cleaner.
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This is best used with fresh stains that haven't previously been cleaned; however, it can be applied to all stains.
Putative ortholog alignments were cleaned using previously reported thresholds [ 63].
Genotypes were cleaned as previously described using standard quality control methods (Paternoster et al. 2012).
Previously, the substrate was cleaned with neutral soap for 10 min in ultrasonic bath, 10 min in distilled water, 10 min in isopropanol, and finally dried with N2.
All steps were cleaned up with Ampure XP beads.
RNA pellets were cleaned further with RNeasy Mini columns (Qiagen).
The measures followed a previously published protocol [8] in which the samples were cleaned with bleach, HCl and water, after which the outer surface was removed.
Ligation reaction material was cleaned with ZR-96 DNA Clean and Concentrtor-5 kit (Zymo Research), as previously described, and eluted in 10 µl of DiH2O.
The microscope plate was cleaned with Iso-propanol and the cryotome surfaces were cleaned with acetone.
The device was cleaned with 10% ethanol after each trial to effectively remove the scent of the previously tested animal.
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