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SDM-25N has been characterized in binding, GTPγS and smooth muscle assays, and has been used in vivo, in brain slices and in cultured cells (Chen et al., 2007; Blomeley and Bracci, 2011; Southern et al., 2013).
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TGF-β1 and TGF-β3 had previously been characterized in terms of binding TβRII and recruiting TβRI using SPR-based methods with immobilized biotinylated TGF-βs.
We first examined existing literature for previously described TREs that had been characterized in vitro for TR binding and TH activation [22], [59] [67].
For instance, LC3-interacting regions have been characterized in ULK1/Atg1 and its binding partners Atg13 and FIP200, which may facilitate LC3 recruitment to PAS [37].
NdgR and its orthologs have been characterized in vitro by DNA-binding experiments [ 15- 18].
Very few, if any, DEAD-box RNA helicases have been characterized in the presence of a binding partner that modulates the ATPase activity [19] and it is interesting to note that the values for KM and kcat for the eIF4AIII-MLN51 SELOR complex are respectively at least 3-and 20-fold higher than those published to date.
RBM10 has been characterized in vitro as an RNA-binding protein and identified as a component of spliceosome complex.
For Dsg 1, Ca2+-dependency of homophilic binding has been characterized in more detail.
Furthermore, multiple transcription factor-binding sites have been characterized in the upstream regulatory region of the MMP-9 gene, including binding sites for the AP-1 and NF-kappaB transcription factors [ 8, 49, 50].
The PtdIns4 P and ARF1 binding sites have been characterized in FAPP1-PH [14].
Some of these motifs have been characterized in animals regarding specificity in the DNA-binding sequence recognition and dimerization activities responsible for the activation or repression of target genes or for binding to small molecules [ 1].
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