The metalized border was changed to standard thread in 1989.
After 4 h the medium was changed to standard differentiation medium.
Viral supernatants were added to the cells for 8 h, the medium was changed to standard growth medium, and the cells were subsequently assayed after 48 h for luciferase activity as described above.
Following 6 h, media was changed to standard culture media containing 5% FBS for further 18 h.
After 72 h, medium was changed to standard culture medium supplemented with 5 μM forskolin, 10 ng/ml basic fibroblast growth factor (b-FGF), 5 ng/ml platelet-derived growth factor AA (PDGFaa), and 200 ng/ml heregulin-β-1 (NRG) (Immunotools, Friesoythe, Germany).
The policy has since been changed to two views as standard at all screens.
The error bars on graphs depicting percentages of phenotypes have been changed to Standard Error of Proportion (SEP) as requested.
After 4 hours transfection with lipofectamine 2000, the medium was changed to the standard medium with antibiotics and the cells were cultured according to the standard protocol.
In the former case the surgical prophylaxis is changed to teicoplanin (standard prophylaxis is cefuroxime and metronidazole), but if it is possible to delay surgery the patient undergoes a course of decolonisation treatment as detailed above, either at home or within the hospital.
"We need access to technologies not only in the refineries to produce high quality fuel but also in the automative sector – a city like Tehran has five million passenger cars and motorcycles and a lot of them are now obsolete, they have to be changed to modern standards vehicles," she said.
Numbering was changed to the British Railways standard numbering system: the series 31810 31875 was allocated to the earlier locomotives, and 31400 31414 to the final 15.
