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This process could create a pattern like the observed one, if our sequencing reactions would have been based on single PCR products of the entire gene.
Many of the previous studies have been based on single or, in some cases, a few samples collected at specific days during each menstrual cycle phase.
Estimates for sequencing effort required for obtaining high (e.g. 90%) coverage within such habitats have been reported [48], [49], but have often been based on single 16S rRNA gene datasets.
Methods of control have usually been based on single major genes that confer complete resistance based upon a gene-for-gene interaction.
Many previous estimates of the staphylococcal phylogeny have been based on single locus gene trees, which in many cases exhibit differing topologies.
Molecular analyses to date show no evidence to support the classification scheme developed from the R&F analyses, but most molecular studies have been based on single genes [see [ 16, 20, 26, 27]].
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Although the burden of MRSA infection has not been systematically estimated nationally, past estimates have been based on single-center or selected population-based studies in the United States.
Management of thymoma has largely been based on single-institution retrospective, observational studies.
However, published estimates vary widely and have been based on single-center or local data with limited applicability (4, 7).
However, most studies are based on single cases with a focus on only one disease or patient group.
"On the other hand," she continued, "his definition of dependence is based on single symptoms, which may be open to question".
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