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In order to get high energy and power densities, an asymmetric supercapacitor has been assembled by using mesoporous nickel cobaltite-graphene nanocomposite as the positive electrode and doped carbon as the negative electrode.

The Tol2-based kdrl Ubiad1-2A-mCherry construct has been assembled by using the Tol2 kdrl Ubiad1-2A-mCherry gateway recombination cloning strategy (kdrl Ubiad1-2A-mCherry

Generation of the Zebrafish Transgenic Line Tg kdrl Ubiad1-2A-mCherry) uTg kdrl Ubiad1-2A-mCherryad1 EndoTg kdrl Ubiad1-2A-mCherryffect The Tol2-based kdrl:utoad1-2A-mCherry construct has been assembled by using the Tol2 Kit and three-fragment gateway recombination cloning strategy (Kwan et al., 2007 ).

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More than 725,000 trimmed reads were assembled by using de novo assembly and analyzed according to BLAST searches (Technical Appendix Figure 1 and Table 1) (6, 7 ).

Raw sequence data were trimmed to remove any primer-derived and low-quality sequence; gene sequences were assembled by using a viral assembly tool (www.jcvi.org/cms/research/software).jcvi.org/cms/research/software

More than 234,000 trimmed reads were assembled by using de novo assembly in CLC Genomics Workbench 4.5.1 (CLC Bio, Aarhus, Denmark) and analyzed according to nucleotide and translated nucleotide BLAST searches (http://blast.ncbi.nlm.nih.gov/Blast.cgi).nih.gov/Blast.cgi

Pryosequencing reads representing 130 Mb of data (ca. 20.4× coverage) were assembled by using the Newbler assembler version 1.1.03.24 (Roche) into 1276 contigs with an N50 contig size of 8455 bp.

The shotgun sequences were assembled by using the GS de novo assembler.

The paired end reads were assembled by using a de novo genome assembly program Velvet version 0.7.03 (19 ), and a multicontig draft genome was generated for each sample.

The clean reads of each gene detected in RNA-seq sequencing analysis was assembled by using Trinity software (de novo assembly) [ 40] and the assemble results were provided in supplemental files.

The sequence reads were assembled by using the Edena de novo short-reads assembler (Genomic Research Laboratory, Geneva, Switzerland).

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