Selected miRNAs that were differentially expressed in senescence were assayed for expression in quiescent cells to identify miRNAs that are specifically associated with senescence-associated growth arrest.
Total CD34+ cell count was also determined, and CD34+ or CD34+KDR+ cells were assayed for expression of CXCR4.
pRBCs were assayed for expression of the varO-antigen which mediates the binding of trophozoites to npRBCs in the rosette.
To validate the microarray expression data, A549 (EGFR wt), PC9 (EGFR delE746-A750), and H1650 (EGFR delE746-A750) were assayed for expression of ZEB1.
BMECs were then purified by subculturing onto 96-well plates, and the cells were assayed for expression of selected BBB and vascular markers.
Cells exposed to the bacteria are subsequently transformed with the sequence of interest and can be assayed for expression a few days after infiltration.
Embryos were assayed for expression of Mooccu.Hand-r (green— A, D, G ) and Mooccu.Foxf (red— B, E ) or Mooccu.Aldh1a (red— H ) by double in situ hybridization.
Thirty-six genes were assayed for expression via qRT-PCR in 20 OSA samples to both correlate array data to qRT-PCR as well as explore potential biomarker expression via a method not subject to multiple sampling errors.
Transgenic plants were assayed for expression of the gus gene that codes for β-glucuronidase (GUS) following the histochemical staining procedure described by Jefferson et al. [ 33] with some modifications.
DOI: http://dx.doi.org/10.7554/eLife.07939.005 To identify effects on fidelity of start codon selection, the mutant strains were assayed for expression of otherwise identical HIS4-lacZ reporters containing an AUG or UUG start codon.
